Refinement of a vitrification protocol for protocorm-like bodies of Dendrobium sonia-28

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Catalase and superoxide dismutase activities and the total protein content of protocorm-like bodies of Dendrobium sonia-28 subjected to vitrification.

Dendrobium sonia-28 is an important ornamental orchid in the Malaysian flower industry. However, the genus faces both low germination rates and the risk of producing heterozygous progenies. Cryopreservation is currently the favoured long-term storage method for orchids with propagation problems. Vitrification, a frequently used cryopreservation technique, involves the application of pretreatmen...

متن کامل

Effects of organic additives and different carbohydrate sources on proliferation of protocorm- like bodies in Dendrobium

The purpose of this study was to investigate the effect of the addition of different organic additives and sugar types as carbon source on the growth and proliferation of protocorm-like bodies (PLBs) of a very new Dendrobium hybrid known as D. Alya Pink (DAP). PLBs were supplemented with homogenates of banana, tomato, and coconut water at various concentrations. Proliferation rate was recorded ...

متن کامل

Effect of Banana Extract on Growth and Development of Protocorm Like Bodies in Dendrobium sp. orchid

An experiment was carried out in Plant Tissue Culture Laboratory, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh to investigate the effect of banana extract on micropropagation of Dendrobium sp. var. Sonia orchid through PLBs. The experiment was conducted during July 2012 to October 2013. Half-Murashige and Skoog (1/2MS) medium were used as basal medium and the medium...

متن کامل

Preliminary Factors Influencing Transient Expression of Gusa in Dendrobium Savin White Protocorm-like Bodies (PLBs) Using Agrobacterium-mediated Transformation System

A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...

متن کامل

Ammonium to Nitrate Ratio Affects Protocorm Like Bodies PLB Formation In vitro of Hybrid Cymbidium

Carbon and nitrogen are the two elements that most affect plant organogenesis. In vitro, usually as part of the macronutrients. Some anecdotal evidence from the literature suggests that the ammonium (NH4+) to nitrate (NO3–) ratio may affect orchid organogenesis. In this study, to test this hypothesis, different NH4+: NO3– ratios were tested on the development of protocorm-like bodies (PLBs) of ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: TURKISH JOURNAL OF BOTANY

سال: 2013

ISSN: 1300-008X,1303-6106

DOI: 10.3906/bot-1208-35